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Bacterial DNA was extracted from infant fecal samples and analyzed by shotgun metagenomic sequencing to profile gut microbiome at the genus level. Correlations are shown between the relative abundance of bacterial genera and fecal IgA against (A) EHEC, (B) Salmonella , and (C) Shigella .

Journal: bioRxiv

Article Title: Discovery of Novel Probiotic Species to Improve Infant Health

doi: 10.1101/2025.10.27.684727

Figure Lengend Snippet: Bacterial DNA was extracted from infant fecal samples and analyzed by shotgun metagenomic sequencing to profile gut microbiome at the genus level. Correlations are shown between the relative abundance of bacterial genera and fecal IgA against (A) EHEC, (B) Salmonella , and (C) Shigella .

Article Snippet: To prepare bacterial DNA samples for microbiome analysis, 100 mg of frozen fecal material was placed in Fast Prep Lysing Matrix D tubes containing ceramic beads (MP Biomedicals) and incubated in 1 mL of PBS on ice for 1 hour.

Techniques: Sequencing